Complete Human Skin Grown From Stem Cells

Complete human skin epidermis has been successfully grown skin-derived stem cells on laboratory mice. This advance could lead to production of one's own skin (an autologous transplant) without the immediate need to harvest the skin from other areas of the body.


(Complex structure of human epidermis)

Background
Cell therapy for large burns is dependent upon autologous epidermis reconstructed in vitro. However, the effectiveness of current procedures is limited by the delay needed to culture the patient's own keratinocytes. To assess whether the keratinocyte progeny of human embryonic stem cells (hESCs) could be used to form a temporary skin substitute for use in patients awaiting autologous grafts, we investigated the cells' capability of constructing a pluristratified epidermis.

Methods
hESCs from lines H9 and SA01 were seeded at least in triplicate on fibroblast feeder cells for 40 days in a medium supplemented with bone morphogenetic protein 4 and ascorbic acid. Molecular characterisation of cell differentiation was done throughout the process by quantitative PCR, fluorescence-activated cell sorting, and immunocytochemical techniques. Keratinocyte molecular differentiation and functional capacity to construct a human epidermis were assessed in vitro and in vivo.

Findings
From hESCs, we generated a homogeneous population of cells that showed phenotypic characteristics of basal keratinocytes. Expression levels of genes encoding keratin 14, keratin 5, integrin α6, integrin β4, collagen VII, and laminin 5 in these cells were similar to those in basal keratinocytes. After seeding on an artificial matrix, keratinocytes derived from hESCs (K-hESCs) formed a pluristratified epidermis. Keratin-14 immunostaining was seen in the basal compartment, with keratin 10 present in layers overlying the basal layer. Involucrin and filaggrin, late markers of epidermal differentiation, were detected in the uppermost layers only. 12 weeks after grafting onto five immunodeficient mice, epidermis derived from K-hESCs had a structure consistent with that of mature human skin. Human involucrin was appropriately located in spinous and granular layers and few Ki67-positive cells were detected in the basal layer.

Interpretation
hESCs can be differentiated into basal keratinocytes that are fully functional—ie, able to construct a pluristratified epidermis. This resource could be developed to provide temporary skin substitutes for patients awaiting autologous grafts.

Artificial skin is a science fiction staple; see the surrogate skin from Robert Heinlein's 1951 novel The Puppet Masters and art-derm from Philip K. Dick's 1961 novel Dr. Futurity and the more functional read-out skin from John Varley's 1992 novel Steel Beach.

Looking for more real-life artificial skin alternatives? I've got you covered:

From The Lancet via medGadget.

Scroll down for more stories in the same category. (Story submitted 12/3/2009)

Follow this kind of news @Technovelgy.

| Email | RSS | Blog It | Stumble | del.icio.us | Digg | Reddit |

Would you like to contribute a story tip? It's easy:
Get the URL of the story, and the related sf author, and add it here.

Comment/Join discussion ( 0 )

Related News Stories - (" Medical ")

Will The FDA Approve This Antiaging Drug?
'So what we're looking for now is... an anti-agathic, an anti-death drug.' - James Blish, 1951.

EVE Artificial Womb For Lambs (For Now)
'In the crimson darkness, stewing warm on their cushion of peritoneum and gorged with blood-surrogate and hormones...' - Aldous Huxley, 1932.

Bees Royal Jelly Helps Wounds Heal Faster
'An alien drug... used by an insect race.' - Clifford Simak, 1961.

3D Printed Bionic Chinese Skin
Designer skin for everyone!

 

Google
  Web TechNovelgy.com   

Technovelgy (that's tech-novel-gee!) is devoted to the creative science inventions and ideas of sf authors. Look for the Invention Category that interests you, the Glossary, the Invention Timeline, or see what's New.

 

 

 

 

 

Current News

Biggest HiSeas 'Mars Mission' Problem? No Internet
I think sf writers have this covered!

Clever Electric Truck Generates More Power Than It Uses
Better than a fictional electrotruck!

Eden-ISS, Greenhouse In Antarctica
'With this kind of light we could get the gardens going again."

Make Space Tools On The Spot (Like Moties)
'A moment ago it was squeezing silver toothpaste in a ribbon...'

Will Robots Be Moral If We Raise Them Like Our Children?
'The birth of Machine, my robot child...'

Foldable Galaxy Phones, I Swear They're Coming (Maybe)
How hard can it be?

Bacteria Behave Differently In Space
'The Republic struggled to control its Sours...'

Brain Connected To Internet - ‘Brainternet'
Fascinating!

Artificial Spider Silk
You can also use it to make a roof - on an asteroid.

MIT Tunes Ions For Frictionless Surface - Superlubricity!
'My telelubricator here neutralizes the interatomic bonds the surface of any solid...'

Seiko Astron Always Knows Your Time Zone
'Harrington glanced at his wrist watch - a bulky affair - and whistled.'

Robot Buddhist Priest Chants, Drums
'He crossed the waiting room to the Padre booth...'

Koniku Kore, Mouse Brain-Based Chip, Detects Explosives
'As a matter of fact, this mouse is going to keep on thinking forever.'

CNH Industrial Autonomous Tractor Concept Video
'...the tiny red glints of self-guided tractors.'

The Neuroon Open Sleep Tracker For Lucid Dreaming
'Leads trail away from insertion points on her face and wrist... to a lucid dreamer on the bedside shelf.'

Siri Now Smoother, Perkier (Thanks, Deep Learning!)
'Good morning, Dr. Chandra. This is Hal.'

More SF in the News Stories

More Beyond Technovelgy science news stories

Home | Glossary | Invention Timeline | Category | New | Contact Us | FAQ | Advertise |
Technovelgy.com - where science meets fiction™

Copyright© Technovelgy LLC; all rights reserved.